Composite

Part:BBa_I712083:Design

Designed by: Marko Bitenc   Group: iGEM07_Ljubljana   (2007-10-22)


CMV promoter - CD4 (shortened) - TEV C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1263
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1876
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1710
    Illegal SapI.rc site found at 798
    Illegal SapI.rc site found at 1915
    Illegal SapI.rc site found at 2225


Design Notes

CD4 (shortened) is also fused with C-terminal half of TEV protease. This construct is coexpressed with CCR5 receptor fused with N-terminal half of TEV protease (in mammalian cells). When that kind of human cells are infected with HIV virus both halves of TEV protease assemble and split protein regains its function. This principle can be used for fighting HIV infection.


Source

Human CD4 is a member of the immunoglobulin superfamily.

References